Nembutal) is a barbiturate used in research as an anesthetic in many animal models. The injectable form of this drug has lately become difficult to procure and prohibitively expensive. Also, Due to this lack of availability, researchers have begun to compound injectable sodium pentobarbital. Some oversight agencies have object to this practice. Claiming a lack of quality control and degradation of the drug. We sought with this study to establish both.

1) a protocol for the preparation of injectable sodium pentobarbital.

2) standard operating procedures to monitor the quality of the preparation and degradation of the drug over time.

Methods

Our preparation consists of a mixture of sodium pentobarbital in alkaline aqueous solution, propylene glycol, and ethanol. Pentobarbital content in this preparation was assay by high-pressure liquid chromatography (HPLC). We also assay pentobarbital content over time in preparations of various ages up to 6 years old.

Results

Furthermore, We determine that the drug degrades at a maximum of 0.5% per year in our preparation (alkaline water/propylene glycol/ethanol) when store in the dark at room temperature. A yellow discoloration develope after about 2 years, which we have arbitrarily determine disqualifies the preparation from use as an anesthetic. Attempts to spectroscopically assay this discoloration were not successful.
Chemicals
Pentobarbital sodium (CID: 14075609)

Introduction

Compounds which exert sedative (reduce anxiety with no effect on motor function) or hypnotic (encourage a near-normal state of sleep) effects have been know since antiquity. Also, These compounds have a variety of different chemistries; alcohols (ethanol), ethers (diethyl ether, paraldehyde) and various plant extracts (opium, cannabis, valerian) have all been use at various times as sedatives/hypnotics. The use of barbiturates for this purpose began with the discovery in 1903 that barbital (diethylbarbituric acid) was effective at putting dogs to sleep (Fischer & Von Mering, 1903). Bayer and Co. did market barbital as veronal in 1904 for use as an anesthetic. Then By modifying the side chains and other ring substituents, other barbiturates have been develop which are longer- or shorter-acting, or have other specific properties related to anesthesia that make them desirable in specific circumstances

Sodium pentobarbital was synthesize and introduce as an oral anesthetic by Abbott Laboratories in 1930. Injectable forms were introduced later, and there were even suppositories for use in children fearful of examination. The drug also enjoys a period of popularity as a treatment for schizophrenia and anxiety. Today, sodium pentobarbital is use clinically as a pre-operative sedative, in certain forms of severe insomnia, and for the emergency management of seizures. Buy Nembutal Powder Online in Germany